withania coagulans extract attenuates histopathological alteration and apoptosis in rat brain cortex following ischemia/reperfusion injury

Authors

maryam sarbishegi cellular and molecular research center, zahedan university of medical sciences, zahedan, ir iran; department of anatomy, school of medicine, zahedan university of medical sciences, zahedan, ir iran

zahra heidari infection diseases and tropical medicine research center, zahedan university of medical sciences, zahedan, ir iran; department of histology, school of medicine, zahedan university of medical sciences, zahedan, ir iran; infection diseases and tropical medicine research center, zahedan university of medical sciences, zahedan, ir iran. tel: +98-33295728, fax: +98-33295728

hamidreza mahmoudzadeh sagheb infection diseases and tropical medicine research center, zahedan university of medical sciences, zahedan, ir iran; department of histology, school of medicine, zahedan university of medical sciences, zahedan, ir iran

abstract

results the i/r group showed a significant increase in pycnotic (dying) neurons and pretreatment with wc at doses of 500 mg/kg and 1000 mg/kg significantly reduced pycnotic and tunel positive neurons, in a dose dependent manner in ischemic brain cortex. conclusions our findings indicated that wc has neuroprotective effects and is able to reduce histopathological alterations and apoptosis in brain cortex i/r in rats. materials and methods thirty-two adult male wistar rats weighing 280 - 300 g were used in this study. animals were randomly divided to four groups (n = 8) as follow: sham operated group (i), i/r group (ii), wce500 + i/r (iii) and wce1000 + i/r groups (iv). pretreatment with wc extract (500, 1000 mg/kg) was done by oral gavage for 30 days and global brain ischemia was induced by the common carotid occlusion for 30 minutes. after 72 hours, the animals were perfused transcardially and then the brains were prepared for histological study (h & e and tunel staining). objectives the aim of this study was to investigate the neuroprotective effects of withania coagulans (wc) extract on brain cortex in a rat model of i/r. background cerebral ischemia and reperfusion (i/r) is a pathological condition that arises by reduction or cessation in cerebral blood flow and return of oxygen and metabolites to brain cells, which cause oxidative damage.

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Journal title:
gene, cell and tissue

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